This is the STARTER LABORATORY INSTRUCTION MANUAL/Guide! It is a LIST for Your 1st Laboratory Tests & Experiments:
NOTE: During the first week you will be given to sharre a LOANER set & short TERMS WILL BE PROVIDED WITH A SET OF LAB CARDS for the 5 week term...
LAB PRACTICAL 1 EXAM MATERIAL:
Please read this page. The Laboratory Practical 1 Exam 1 is 20%+ of your final grade. It is composed of the lecture and video material (Chapters 1, 2 (on your own), 3, 4, , 9 and the following Lab material. You must be able to handle microbes aseptically and in a standard and careful manner (SAFETY CARD). You must be able to discuss, perform, recognize and explain the following PROCEDURES: Cleaning a Slide, Smear from Agar, Smear from Broth, Negative/Capsular Stain, Simple Stain and the Differential Stains: *Gram, Acid Fast, and Endospore. Please make Lab Guide FIRST!
*=SUPER IMPORTANT!!!!
MIDTERM/LAB PRACTICAL 2 EXAM MATERIAL:
Please read this page. The EXAM 2 is the MIDTERM/LP2 EXAM (20%) is the most important exam and most likely predictor of your Final Class Grade. It is composed of the lecture and video material from Chapters: 5, 6, 7, 8 and all the Lab Manual that you make!
This LAB MANUAL is 50% of the MIDTERM QUESTIONS. The midterm exam is composed of 200 multiple choice questions and a WORDGAME crossword puzzle-type questions which use data and the dichotomous key (listed below) to determine the correct Genus, just as you would do in class. This exam covers all the Lab Guide (50%) and the lecture topics listed below. This exam counts 20% of your Final Grade. YOU DO NOT HAVE TO MEMORIZE the dichotomous key - it will be provided during the MT exam. Your are required to SHOW your work on the WORDGAME questions and you must attempt at least one of these type questions to pass the midterm exam.
The lectures topics/chapters covered in MT/LP2 exam are: Chapters 4, 5, 6, 7, 8, 9. The order covered is: Chapter 8, 4, 5, 6, 7, and 8. See the powerpoints for the lecture notes. Several videos are also included and all of the LAB Guide is on the exam 2!
Click on the Experiments will contain 5 BOLDED headings:
Chemistry, Inoculation, Incubation, Evaluation, and Comments/Cautions.
There are 2 reasons for earning these Lab GUIDE
(1) You need a STEP by STEP Lab Manual with the steps you are to perform in lab listed neatly and in a large easily read "font" on one page to lay on the counter as you perform these tests and procedures on your Unknown microbe in laboratory class... THIS IS YOUR LAB GUIDE, and since only the minimum is printed in the Guide it will be easier to learn. My idea is to organize by types of media - ask me in class, how?
(2) You need something short and concise to use to memorize for the 100 multiple choice questions you will have from this material that is on the MIDTERM Exam.
THINGS TO REMEMBER ABOUT MAKING YOUR LAB MANUAL/CARDS!
- Knowing these unknown tests helps your MIDTERM GRADE. bring you loaner Lab Guide to every class
- YOUR VIDEO notes Click here for a link to early lab videos to watch:BONUS are covered in the test/exam questions.
- The purpose of this exercise to give the student a reward for preparing a study aid... AND, to provide said study aid for use at least two weeks prior to the most difficult exam - The Midterm Exam. The MIDTERM EXAM counts 20% of your class grade and includes all the lecture Unit II material on Biochemistry with an emphasis on enzymes. Additionally, it covers all of the UNKNOWNS material in the Microbiology Laboratory Online section...
- The SMEARS + STAINS Cards as well as the chapter on the Microscope are the source of the questions for Lab Practical 1 which is a demonstration of how to perform the major "tasks" of Microbiology Lab.
- In the past we had you PRINT/MAKE THIS LAB MANUAL or FLASH CARDS yourself as it INVOLVES THOUGHT AND PLANNING - AND!!!! It's best to put the material in your own words! If you just "COPY" THE MATERIAL FROM THE ATLAS OR WEBSITE ONTO A CARD you won't learn it!!!! You may however, abbreviate some repeating items... such as LOOP SLANT or LOOP BROTH or LOOP LINE inoculate or even Loop/Stab inoculate for a Slant/Deep...
- You may work in groups to LEARN the cards/TESTS
- THESE CARDS ARE YOUR LABORATORY PROCEDUREs and STUDY MANUAL - you will use them in the class lab
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HERE IS THE INFO YOU WISH TO CONVEY...
CHEMISTRY - these tests are fermentation reactions to see if and what carbohydrates your microbe can use for "food" and thus produce an acid (or alcoholic+ gas) waste (with gas or without) detected by the pH indicator Phenol Red that is + or yellow when the pH falls below 6.8. There can me innumerable different carbohydrates made this way and agar in a slant-deep may also be used instead of saline. The carbs used in our course include Phenol Red: glucose (Dextrose old name), lactose, sucrose, saccharose, dulcitol, and mannitol. Others may be added during certain terms.
INOCULATION - it is a liquid or BROTH so LOOP DIP
INCUBATION - incubate according to the Optimum Temperature test for 7 days (remember color change = 7 days) checking for results the 1st DAY and every other day
EVALUATION - observe every other day by taking it from the incubator and mixing and holding it against white paper. Yellow = Positive but! Pink, orange, and red = negative... note: if negative BEFORE 18 hrs put it back in the incubator until 7 days (re-incubate)! Results = Fast Post, Mod Fast Pos, Slow Pos, Negative, and if positive = with gas or without (look for bubble the inside up-sides-down Durham tube. Note: there is an exception where alcoholic fermentation is seen at 7 days and the tube appears whitish-pink with gas.
CAUTIONS/COMMENTS - be careful to NOT confuse a very small bubble in the Durham tube for GAS, the bubble must be substantial... And, it cannot have GAS if it is NOT POSITIVE (unless it is Alcoholic)! When reading the first time AFTER over 18hrs for the first observation your results are UNRELIABLE due to REVERSION (see Atlas).
INOCULATION - it is a liquid or BROTH so LOOP DIP
INCUBATION - incubate according to the Optimum Temperature test for 7 days (remember color change = 7 days) checking for results the 1st DAY and every other day
EVALUATION - observe every other day by taking it from the incubator and mixing and holding it against white paper. Yellow = Positive but! Pink, orange, and red = negative... note: if negative BEFORE 18 hrs put it back in the incubator until 7 days (re-incubate)! Results = Fast Post, Mod Fast Pos, Slow Pos, Negative, and if positive = with gas or without (look for bubble the inside up-sides-down Durham tube. Note: there is an exception where alcoholic fermentation is seen at 7 days and the tube appears whitish-pink with gas.
CAUTIONS/COMMENTS - be careful to NOT confuse a very small bubble in the Durham tube for GAS, the bubble must be substantial... And, it cannot have GAS if it is NOT POSITIVE (unless it is Alcoholic)! When reading the first time AFTER over 18hrs for the first observation your results are UNRELIABLE due to REVERSION (see Atlas).
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You may use Bergey's Determinative (6, 7, 8, &/or 9th), Bergey's Systematic (1st or 2nd), MacFaddin (2nd or 3rd) or any respected reference. The reference that most matches YOUR results should be your first column ref.
CLICK HERE FOR MORE WORD PROBLEM HINTS PAGE 2
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| HINTS ON TYPING THE UNKNOWN REPORT |