STAIN GAME ANSWERS 8/22/22

NEW ANSWERS - STAIN GAME! 

A - Endospore positive large pink rod cells (remember that makes it Gram Positive & Acid Fast Negative by memorization) with holes and lots of green oval endospores (not cells) which take up the malachite green when cooked because endospore coats are hard to stain like lipids and capsules. ONLY BACILLUS & CLOSTRIDIUM are ENDOSPORE POSITIVE.
B - Acid Fast (AF positive & remember AF+ is Gram positive) small hot pink and baby blue vegetative rods (meaning cells and thus not endospores) that are 2 different colors but the same size; some of the cells' lipids got enough HEAT to take the carbolfuchsin and turned hot pink and some didn't and stayed baby-blue this is MYCOBACTERIUM as only Mycobacteria & Nocardia are AF positive
C - Endospore negative small pink and green rods the same size; the pink rods are too small to have "hole" and if you could see them, they don't. also the green items are NOT oval, they are rods. When cooking some of the lipid covered rods took the malachite green and some didn't, they took the pink; MYCOBACTERIUM again using the Endospore differential stain
D - Acid Fast (AF positive) small hot pink and baby blue vegetative rods (meaning cells and thus not endospores) that are 2 different colors but the same size; some of the cells' lipids got enough HEAT to take the carbolfuchsin and turned hot pink and some didn't and stayed baby-blue this is MYCO-BACTERIUM.  The reason that #B above and this one #D look a bit different is how hot the primary stain was cooked; the hotter the more hot pink. Remember, that you must have small rods and at least 1 hot pink & 1 baby-blue of the same size to be AF positive NOT HOT PINK OVALS!
E - Non-acid Fast (AF negative) large baby-blue vegetative rods with hot pink endospores; this is BACILLUS. THE CELLS STAIN BABY-BLUE; endospores are NOT cells - only DNA inside a Ca++ ion spore coat with no other cell parts - ENDOSPORES ARE NOT ALIVE
F - Gram positive small rods (actually Gram variable on the positive side); this is MYCOBAC-TERIUM again using the Gram different differential stain
G - Endospore positive large pink rod cells (remember that makes it Gram Positive & Acid Fast Negative by memorization) with holes and 1 or 2 green oval endospores (not cells) which take up the malachite green when cooked because endospore coats are hard to stain like lipids and capsules. ONLY BACILLUS & CLOSTRIDIUM are ENDOSPORE POSITIVE.  THIS STAIN COULD ALMOST BE GRAM NEGATIVE LARGE RODS WITH HOLE BUT!  There is some visible green GARBAGE VIABLE WHICH MAKES IT AN ENDOSPORE STAIN
H - Gram positive large hot dog-shaped rods with lots of pink irregularly shaped pieces and oval pink endospors....MAJORITY COLOR THEN, IF NO SURE "WHAT IS ON TOP"
I - Gram positive cocci
J- Non-Acid Fast (AF-) baby blue large rods; see holes?what does that mean is the result for this microbe on Gram and Acid Fast staining?
K- Gram positive large hot dog-shaped rods with faint oval pink endospores....MAJORITY COLOR THEN, IF NO SURE "WHAT IS ON TOP"
L - Endospore positive large hot dog-shaped pink rods lots of holes and a few oval pink endospores...green garbage seen in upper areas
M & N - Endospore negative very small (!) pink and green rods the same size; no holes; no ovals.  When cooking some of the lipid covered rods took the malachite green and some took the pink; MYCOBACTERIUM again using the Endospore differential stain
O - Acid Fast (AF+) small hot pink and baby-blue rods the same size; MYCOBACTERIUM
P - Gram negative small rods (no green garbage so this is NOT Endospore negative staining)
Q - Acid Fast (AF+) small hot pink and baby-blue rods the same size; MYCOBACTERIUM
R - Negative Stain from teeth
S - Gram positive large rod (see holes?)
T - Endospore positive large hot dog-shaped pink rods lots of holes and a few oval pink endospores.. plus some green garbage
U - same as E above
V - Endospore positive large hot dog-shaped pink rods lots of holes
W - a human Red Blood Cell under 100X
X - Acid Fast (AF+) small hot pink and baby-blue rods the same size; MYCOBACTERIUM
Y - Endospore positive large hot dog-shaped pink rods lots of holes
Z - Endospore negative very small (!) pink and green rods the same size; no holes; no ovals.  When cooking some of the lipid covered rods took the malachite green and some took the pink; MYCOBACTERIUM again using the Endospore differential stain
AA - Gram negative small rods (NO GREEN GARBAGE)
BB - Gram positive cocci
CC - Non-Acid Fast (AF-) small baby-blue rods the same size; E. coli
DD - Acid Fast (AF+) small hot pink and baby-blue rods the same size; MYCOBACTERIUM
EE - Endospore negative very small (!) pink and green rods the same size; no holes; no ovals.  When cooking some of the lipid covered rods took the malachite green and some took the pink; MYCOBACTERIUM again using the Endospore differential stain   MOST PEOPLE MISS THIS 1 OVER AND OVER - DON'T LOOK FOR GREEN FOR ENDOSPORE POSITIVE!